Australian Herbicide Resistance Initiative (AHRI)


Working in a number of research areas, AHRI has produced a large number of publications which are available to download. View the latest publications below, or search with the filter.


Genetic inheritance of dinitroaniline resistance in an annual ryegrass population

The increasing number of weedy species resistant to dinitroaniline herbicides warrants studies on the evolutionary factors contributing to resistance evolution, including genetic inheritance of resistance traits. In this study, the researchers investigated the genetic control of trifluralin resistance in a well-characterised Lolium rigidum Gaud. population from Western Australia. This population was purified to contain plants homozygous for the Val-202-Phe α-tubulin mutation, and used as the resistant (R) parents and crossed with susceptible (S) parents to produce eight reciprocal F1 families.

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Identity and Activity of 2,4-Dichlorophenoxyacetic Acid Metabolites in Wild Radish (Raphanus raphanistrum)

Synthetic auxin herbicides, such as 2,4-dichlorophenoxyacetic acid (2,4-D), are widely used for selective control of broadleaf weeds in cereals and transgenic crops. Although the troublesome weed wild radish (Raphanus raphanistrum) has developed resistance to 2,4-D, no populations have yet displayed an enhanced capacity for metabolic detoxification of the herbicide, with both susceptible and resistant wild radish plants readily metabolizing 2,4-D. Using mass spectrometry and nuclear magnetic resonance, the major 2,4-D metabolite was identified as the glucose ester, and its structure was confirmed by synthesis. As expected, both the endogenous and synthetic compounds retained auxin activity in a bioassay. The lack of detectable 2,4-D hydroxylation in wild radish and the lability of the glucose ester suggest that metabolic 2,4-D resistance is unlikely to develop in this species.

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Rainfall affects leaching of pre-emergent herbicide from wheat residue into the soil

No-tillage with stubble retention is a widely used cropping system for its conservation and yield benefits. The no-tillage farming system in southern Australia relies heavily on herbicides for weed management, but heavy crop residues may have a negative impact on the activity of pre-emergent herbicides applied. Any herbicide intercepted by the crop residue may not reach the soil surface without timely rainfall and may dissipate due to volatilisation, photo-degradation and/or microbial activity. Two experiments were carried out to investigate the interception of prosulfocarb, pyroxasulfone, and trifluralin herbicides by wheat residue and retention following simulated rainfall.

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