Australian Herbicide Resistance Initiative (AHRI)

When on a good thing…

Gregor Mendel

My mother has brown eyes and my father and I have green eyes. How does that work? Answer, Mum was a heterozygote.

No this is not some deadly disease, it simply means that on one chromosome she has the dominant gene for brown eyes, and on the other she has the recessive green / blue eye gene.

Mendelian genetics explains how plants and animals inherit traits from their parents and has been used by AHRI researcher Dr. Roberto Busi to show why it is a good idea to rotate herbicides.

This study showed that Sakura® (pyroxasulfone, Zidua® in U.S.A) resistance is inherited as a single gene. Roberto crossed highly multiple resistant ryegrass with susceptible ryegrass and found that the progeny was less resistant to a range of herbicides than the resistant parent. This was because the progeny were heterozygotes.

It’s a pretty simple message, herbicide rotation is a good idea to prolong the life of herbicides.

Are you struggling to remember the difference between a homozygote and a heterozygote?

We need to start with a spot of revision!

Those of you who studied biology will remember the story of Mendel’s peas.

Gregor Mendel

Gregor Mendel 1822-1884

Mendel crossed peas with yellow seeds with peas that had green seeds. The f1 (first progeny) all had yellow seeds. When he studied the f2 (second progeny) he observed a 3:1 ratio of peas with yellow : green seeds. Is it coming back to you?

Genes in peas generations

The Yellow seed gene (Y) is dominant. In this example the f1 generation are all heterozygotes (“hetero” just means different i.e. YG) and consequently all seeds present as yellow. The f2 generation produces 25% yellow homozygotes (“homo” means the same, i.e. YY), 50% Heterozygotes (YG, yellow seeds), and 25% Green homozygotes (GG, green seeds).

Mendel's model of plant genes through generations

Enter the amazing Roberto Busi.

Roberto Busi

By understanding how Sakura® resistance is inherited, Roberto can demonstrate the benefits of rotating herbicides. In North America pyroxasulfone is marketed as Zidua®, in Australia it is called Sakura®.

Roberto started with ryegrass that was already resistant to trifluralin, metolachlor, sulfonyl urea and ACCase (grass selective) herbicides. It is likely that this resistance is due to enhanced metabolism. He exposed this ryegrass to three recurrent selections with Sakura® (pyroxasulfone) and evolved resistance to Sakura®. He then sprayed these with a high rate of Sakura®. The high rate (double label rate) of Sakura® killed the heterozygous and susceptible ryegrass plants leaving him with only homozygous (RR) plants. These plants were crossed with susceptible ryegrass (SS).

Table showing plants resistance through genes

The really good news was that the heterozygous f1 plants (RS) were more susceptible to Sakura® and a range of other herbicides than the homozygous (RR) parents. The RS plants still possessed some low level resistance but not as much as the homozygous parents.

The graph below shows that the multiple resistant population (MR) has high levels of resistance to five common pre-emergent herbicides (green bars). When this multiple resistant ryegrass was crossed with susceptible ryegrass the progeny (f1, purple bars) were less resistant than the parent (green bars). This was particularly the case for Boxer Gold® (prosulfocarb) where the f1 became very susceptible to this herbicide.

We need to keep in mind that this was achieved in the laboratory and Roberto crossed the resistant plants with plants that were completely susceptible. In the field completely susceptible ryegrass are rare. However, this research does show the value of rotating herbicides, particularly for regions with relatively low resistance levels.

Survival of ryegrass to a range of common pre-emergent herbicides

Figure 1. Survival of ryegrass to a range of common pre-emergent herbicides (see table 1 below for key to herbicides) where 1 refers to 100% survival. Green bars – multiple resistant ryegrass (four selections with Sakura®); Purple bars – f1 (cross between multiple resistant and susceptible ryegrass); Blue bars – susceptible ryegrass (VLR1).

Table 1. Key to herbicides in figure above.
Legend for graph shown above

What does this mean for herbicide rotation in the field?

A single spray with Sakura® in the field is likely to result in some resistant survivors. This small number of survivors are most likely to cross pollinate with the susceptible individuals around them creating heterozygous (RS) plants.

Two options:

1. Don’t rotate herbicide

If this population was sprayed with Sakura® for several years in a row the population would become enriched and end up with many homozygous (RR) plants with high level resistance. These plants are likely to be cross-resistant to the other pre-emergent herbicides such as Boxer Gold® and Triallate.

2. Rotate herbicides

Rotating to an alternative herbicide such as Boxer Gold® is a good idea because, as Roberto has demonstrated, the heterozygous (RS) plants will be controlled with Boxer Gold® and the resistant genes may not persist in the population.

Rotating herbicides is a good idea.

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